Serpent Holes are a method of transportation in Darksiders that War can use to get from one place to another. He can only move between the areas that have Vulgrim's shops, so he is unable to teleport to The Hollows or Eden, as examples. When War chooses to use a Serpent Hole, he is teleported to a floating pathway in what appears to be a different dimension, which he must traverse to reach the portal at the opposite end. Nevertheless, the Serpent Holes are a much quicker way of traveling between locations. There are also some chests available inside Serpent Holes.

The Serpent Holes are only accessible after defeating Tiamat. As there is no other way to leave the Twilight Cathedral, following the death of the angelic beast, Vulgrim allows War use of the Serpent Holes, in exchange for a glimpse of Tiamat's heart.

Serpent Holes are a quick easy way to move across big areas of land. They are formed by platforms that are stationary, and as you move towards the edge of one, pieces of ruined rock that look like puzzle pieces float up and make a bridge.